And to determine the biologic synthesis of the chondrocytes by toluidine blue, alkaline phosphatase staining and type ⅱ collagen immunohistochemistry reaction. 借助特殊染色、免疫组织化学的方法了解糖胺聚糖、碱性磷酸酶、型胶原的合成情况;
The survival of cells was judged by a kind of histochemical staining method, succinate dehydrogenase ( SDH) reaction: If there were even and fine blue SDH granules in the cytoplasm, the cells survived, and were marked as be positive. 判断细胞存活标准采用琥珀酸脱氢酶组织学染色方法:胞浆内存在均匀细小的兰色琥珀酸脱氢酶颗粒,表示细胞存活,记为阳性细胞。
It has been shown that staining reaction was existed, in mucosal epithelia taken from different segments of tract The gastric mucosa had a negative supranuclear region using HRP-PNA staining method. 在各段消化管的粘膜上皮均有染色反应,但胃粘膜上皮细胞的核上区不被PNA着色。
AKP and RNA in liver cells were strong in staining reaction during the early stages of liver development and got weak apparently from 12th day on. 肝脏发育早期,AKP及RNA的染色反应强,第12天后明显减弱。
Alkaline phosphatase staining showed strong positive reaction in cytoplasm. Tetracycline fluorescence label showed positive reaction in the nodules. 胞浆碱性磷酸酶染色强阳性,在一定条件下可产生钙化结节,结节的四环素荧光标记呈阳性。
The cornea fluorescence staining took negative reaction in all 32 cases. 32例患者的角膜荧光染色检查全部呈现阴性,均已不着色。
In gastric glands, the parietal cells showed only positive staining with WGA in human. While chief cells showed positive reaction with WGA and PNA. All lectin reactions with both cells showed positive staining in rat and mouse. 在胃底腺中,人的壁细胞只对WGA反应,主细胞对WGA和PNA都有反应,而大小鼠二种细胞对三种凝集素均有反应。
There was significant species difference of staining reaction in chief cell and parietal cell of gastric glands: both cells showed positive staining in human and dog, while only chief cells showed positive reaction in rat and mouse. 胃底腺内壁细胞和主细胞的反应有明显的种系差异,人和犬二种细胞都为阳性反应,而大小鼠只有主细胞有阳性反应。肠腺阳性反应。
The staining of peritoneal M φ smear was served as a control in which S-100 protein reaction was negative and ACP reaction was positive. 腹腔Mφs贴片对照染色,Mφs呈ACP强阳性反应而S-100蛋白反应为阴性。
A systematic identification including staining, morphology, cultural characters, biochemical properties, serological reaction, pathogenicity, sensitivity to antimicrobial agents and plasma coagulase test was done for all isolates. 对全部分离菌进行了系统鉴定,包括形态、染色、培养特性、生化特性、血清学反应、致病力、对抗菌剂的敏感性和血浆凝固酶试验等。
Results of Bern-hard's technique for RNP ( ribonucleoprotein) preferential staining indicated that the main chemical component of the NR was RNP, and sometimes bleached regions showing the same reaction as chromatin in Bernhard's staining appeared in the structure. Bernhard染色结果表明,核仁残体的主要成分是核糖核蛋白(RNP)颗粒和纤维,一些核仁残体中存在着与染色质染色反应相似的被漂白区。
It was clarified that the cultured cells were originated from stem cells. The adherent cultured cells increased the activity of ALP, ALP staining reaction and calcium nodus alizarin red staining reaction were positive with the osteogenic inducer. 贴壁细胞在成骨诱导剂作用下,碱性磷酸酶(ALP)水平升高,ALP钙钴染色呈阳性反应,钙结节茜素红染色反应阳性。
The study was carried out on the pathogenic bacterial soft rot isolated from tulip bulb. Observation was made on the morphology of pathogenic bacteria staining reaction, optimum temperature and pH of growth and physiological and biochemical properties. 对郁金香软腐病病原细菌进行了形态观察、致病性测定、染色反应、培养性状观察和生理生化反应。
Results The staining reaction of CD 34 was mainly confined to vascular epithelial cells in tumor stroma, whereas that of Ki 67 was mainly to the nucleus in tumor cells. 结果CD34主要表达于宫颈癌巢间质血管内皮细胞,而Ki-67主要表达于宫颈癌细胞核。
The positive staining mainly appeared on nuclear membranes. Positive reaction of whole nucleus occurred in 21 cases ( 44.68%). 阳性主要显示于核膜,全核均有反应者21例(44.68%)。
Their dynamic morphological changes, growth curve, glycerophosphate dehydrogenase pattern, oil red O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the preadipocytes replayed their in vivo hyperplasia and hypertrophy process. 经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红O脂肪染色及对胰岛素和地塞米松反应的测定,证明是功能活跃的前脂肪细胞,并在体外重现了其增殖、肥大的全过程。
By negative staining electron microscope, virions were roughly round and approximately 40~ 60 nm in diameter with peplomers. Density is 1.13~ 1.14g/cm3. The hemagglutination reaction test proved that this virus had no any reaction to erythrocyte of chook, rabbit, pig and sheep. 电镜负染检查可见直径为40 ̄60nm有突起的圆形或近圆形的病毒颗粒,病毒在蔗糖中的浮密度为1.13 ̄1.14g/cm3。与牛病毒性腹泻-粘膜病病毒颗粒相似。
With the fetal age increased, staining reaction of NOS increased gradually and reached its peak at the ninth month. 随着胎龄的增加,NOS染色反应逐渐增强,至第9个月龄时达到阳性表达的高峰。
Alizarin red S staining showed that the reaction of calcifying nodules was positive when cultured for 4 weeks. 细胞培养4周后,茜素红S染色法示结节呈红色钙阳性反应。
Immunohistochemical staining and reverse transcription-polymerase chain reaction ( RT-PCR) were used to detect the expression of the nestin, pancreatic and duodenal homeobox gene-1 ( PDX-1/ IPF-1), islet endocrine hormone insulin and glucagon. 用免疫组织化学和逆转录-聚合酶链反应(RT-PCR)方法检测巢蛋白、胰十二指肠同源盒基因-1(Pancreaticandduodenalhomeoboxgene-1,PDX-1/IPF-1)以及胰岛内分泌激素胰岛素和胰升糖素(Glucagon)的表达。
None of staining reaction was found in the granular cells and cumulus cells. 颗粒细胞和卵丘细胞上未见着色。
Immunohistochemistry method was used to detect the PKC-δ and IKK-β staining reaction of hippocampal tissue. 免疫组织化学法检测了每组大鼠海马组织PKC-δ和IKK-β的免疫染色反应。
Experiments by Akt and Mdr-1 protein immunocytochemical staining can be seen, Mdr-1, Akt in PANC-1 cells showed a brown positive reaction. 由各组实验Akt与Mdr-1蛋白表达免疫细胞化学染色结果可见,Mdr-1、Akt在PANC-1细胞中的阳性表达均呈棕色反应。